Queen Bitch is the ninth chapter of Duke Nukem Forever.
Duke battles the Alien Queen. In order to damage the queen, Pipe Bombs are first bounced off pads on her left and right. The Pipe Bombs must be detonated when they reach the queen's back. When detonated, the queen opens her arms, allowing Duke to unload munitions and to deal damage.
After the queen is defeated, Duke is knocked to the ground. EDF soldiers come to the rescue.
There is one new ego boost on this level that cannot be found on any previous level:
- Alien Queen: Kill the Alien Queen. (+1 Ego)
- When coming face-to-face with the Alien Queen, Duke will say, "Baby, you make me wish I had three guns". This is a reference to Total Recall. It can also be seen as an ingame joke about Duke only being able to carry two guns at a time.